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In addition, histochemical methods require fixation and extensive chemical treatment of samples, creating the strong likelihood for metal redistribution, leaching, or contamination. Specifically, histochemical methods are not uniformly sensitive to all chemical forms of a metal, often displaying variable sensitivity to specific “pools” or chemical forms of a metal. Although classical histochemical methods for metal detection exist, such approaches are typically limited to qualitative analysis. In this study, elemental mapping by XFI was applied to characterise the elemental content within neuronal cell layers of hippocampal sub-regions of mice and rats. XFI has gained widespread use as an analytical technique in the biological sciences, and in particular enables exciting new avenues of research in the field of neuroscience.
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E-mail: b Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia c Department of Anatomy and Cell Biology, University of Saskatchewan, 107 Wiggins Rd, Saskatoon, Saskatchewan S7N 5E5, Canada d Canadian Light Source, 44 Innovation Boulevard, Saskatoon, Saskatchewan S7N 2V3, Canada e College of Pharmacy and Nutrition, University of Saskatchewan, 107 Wiggins Rd, Saskatoon, Saskatchewan S7N 5E5, Canada f School of Public Health, Curtin University, Bentley, WA 6102, Australia g Australian Synchrotron, ANSTO, 800 Blackburn Road, Clayton, VIC 3168, Australia h ARC Centre of Excellence for Advanced Molecular Imaging, La Trobe Institute for Molecular Science, Bundoora, Vic 3089, Australia i Molecular and Environmental Sciences Group, Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada j Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, Saskatchewan S7N 5C9, CanadaĪ unique combination of sensitivity, resolution, and penetration make X-ray fluorescence imaging (XFI) ideally suited to investigate trace elemental distributions in the biological context. Ij a Curtin Institute for Functional Molecules and Interfaces, School of Molecular and Life Sciences, Curtin University, GPOBox U1987, Bentley, WA 6845, Australia. Metallomics, 2019, 11, 151-165 Elemental characterisation of the pyramidal neuron layer within the rat and mouse hippocampus †